李石磊，张阳阳，关明，杨慧，魏妍波，赵镇文*
单位
1．中国科学院化学研究所活体分析实验室，北京质谱中心
2．中国科学院大学基金项目:国家自然科学基金(21575146，21321003，21405160)
中文摘要:脂质组学概念自2003年被提出以来，其已成为研究生物体、组织或细胞中脂质的结构、功能及代谢途径的一门学科。脂质的种类众多，同时结构非常复杂，脂质的分析充满了困难和挑战。基质辅助激光解吸电离质谱成像(MALDI MSI)分析技术不仅可以进行物质鉴定，而且可对被分析物进行空间分布成像，近年来，该技术广泛地应用于脂质组学的研究。该文介绍了MALDI MSI在脂质组学研究中的样品处理、基质喷涂及应用方面的研究进展，并就目前存在的问题及解决方案进行了探讨，以期扩展MALDI MSI的应用范围。
中文关键词:基质辅助激光解吸电离质谱成像；脂质组学；样品制备；基质；标志物 综述
Application of MALDI MSI in Lipidomics Research
Abstract:Lipidomics,firstly proposed in 2003,is an emerging field, where the structures, functions and dynamic changes of lipids in cells,tissues or body fluids are investigated.Studies have showed that the disturbance of lipid metabolism is closely related to some diseases,such as cancer,atherosclerosis,diabetes,obesity and Alzheimer disease.Therefore, the comprehensive analysis of lipid becomes significant. However, due to the diversity and complexity of lipids,lipids analysis is still full of challenges.Recently,Matrix-assisted laser desorption and ionization mass spectrometry imaging(MALDI MSI) has been developed quickly in lipidomics research. Compared with traditional LC-MS,MALDI MSI provides spatially resolved ion intensity maps corresponding to the mass-to-charge ratio of intact molecular species at specific locations in a prepared tissue sample, which is very useful in biomarker identification analysis,drug distribution analysis,etc. MALDI MSI is a relatively new technique,it still has many aspects needing further improved,such as small molecules detection, neutral lipids detection,ion suppression elimination,detection sensitivity improvement,etc.In MALDI MSI analysis,sample preparation,new matrix development and application research are the three hotspots.This review aims to introduce the research progress of MALDI MSI in sample processing,matrix coating and its application.The existing problems and possible solutions are discussed,in order to extend the application of MALDI MSI.
Key Words:matrix-assisted laser desorption/ionization mass spectrum imaging(MALDI MSI) lipidomics sample preparation matrix marker review
引用本文：李石磊，张阳阳，关明，杨慧，魏妍波，赵镇文*.MALDI MSI在脂质组学研究中的应用[J].分析测试学报,2017,36(2):170-177.
