苏加坤，应旭辉，罗娟敏，王义明*，徐达，罗国安，蔡继宝*
单位
1.江西中烟工业有限责任公司
2.珠海清大弘瑞生物科技有限公司3.清华大学化学系
基金项目:中国烟草总公司重大专项项目(110201401025(JH-03))
中文摘要:应用代谢组学方法，比较研究了普通卷烟和含有天然本草添加剂卷烟对大鼠肺组织代谢的影响。运用液相色谱-质谱联用技术分别分析了对照组、普通卷烟及某品牌天然本草添加卷烟烟气暴露7,14,30 d时的大鼠肺组织样品，对所得数据用偏最小二乘判别分析(PLS-DA)进行模式识别并筛选生物标志物。结果显示，吸食普通卷烟和某品牌卷烟，均会损伤大鼠肺组织，造成磷脂代谢、脂肪酸代谢紊乱，其中有2只普通卷烟暴露组大鼠的肺组织样品显著异常，受到明显的氧化损伤。综合来看，特别是长时间烟气暴露状态下，吸食某品牌卷烟造成的肺组织损伤低于普通卷烟，表明烟草中添加天然本草在一定程度上可减轻烟气对机体的损伤。
中文关键词:液相色谱-质谱联用 代谢组学 天然本草添加卷烟 肺组织 生物标志物
Lung Metabolome Research of Rats Exposed to Tobacco Smoke with Herbal Additives by Liquid Chromatography-Mass Spectrometry
Abstract:The effect of tobacco with herbal additives on the global metabolome of rat lung was studied using liquid chromatography-mass spectrometry(LC-MS).Lung samples from control rats，normal tobacco smoked rats and a certain commercial tobacco(a specific tobacco with herbal additives)smoked rats were analyzed，and the groups were exposed to smoke for one week，half month and one month，respectively.The data were processed by the method of partial least squares discriminant analysis(PLS-DA).The potential biomarkers were screened out according to VIP(Variable Importance in the Projection) and identified by database and standards.The PLS-DA results of different tobacco exposed periods showed that the metabolism of both the normal tobacco exposed group and the certain commercial tobacco exposed group were affected，mainly including phospholipid metabolism and fatty acid metabolism. In particular，two lung samples from rats exposed to normal tobacco were abnormal significantly as a result of suffering from a serious oxidative damage. Overall，especially prolonged exposure to smoke，the smoke damage of the certain commercial tobacco smoked rats was lower than that of the normal tobacco smoked rats，indicating that adding some herbal additives to tobacco could contribute at least partly to reduce the harm caused by smoking.
Key Words:liquid chromatography-mass spectrometry(LC-MS) metabonomics tobacco with herbal additives lung biomarker
引用本文：苏加坤，应旭辉，罗娟敏，王义明*，徐达，罗国安，蔡继宝*.天然本草添加卷烟烟气暴露大鼠肺组织代谢组学研究[J].分析测试学报,2016,35(12):1521-1527.
